Journal Articles

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A combined DFT and experimental study of proline/β-cyclodextrin inclusion complex
(2016-01-25) Zaboub,A.; Madi,F.; Merdes,R.; Mohamedi,M.; Nouar,L.
The binding of the L-proline (Pro) amino acid with β-cyclodextrin (β-CD) was investigated theoretically by B3LYP/3-21G+ method and experimentally by UV–visible and FTIR techniques. Two modes of complexation were considered for studying such complex; in A complex the pyrrolidine ring of the guest was directed toward secondary hydroxyls of β-CD, while in B complex the pyrrolidine was directed toward primary one. Energetic analysis indicates that the formed complexes are stable and both host and guest were deformed after complexation. Electronic properties given by TD-DFT calculation and NBO analysis clearly demonstrate that a charge transfer was occurring between Pro and β-CD molecules.
A Study of Parasitic Copepod Infesting Two Freshwater Fish Populations (Cyprinus carpio and Abramis brama) from Beni-Haroun Dam (Mila) North-East of Algeria
(2020-02-12) Berrouk,H; Tolba,M; Touarfia, M; Boualleg,Ch.
Copepods are one of the main fish ectoparasites, commonly known among the parasitism world, due either to their special adaptive capacity or to induce stress in aquatic organisms, as well as the parasitism result is overall a physiological rate affecting the survival of host. This study was, therefore, aimed to investigate the ectoparasite copepods, infesting 277 fishes including 135 of Cyprinus carpio (Linnaeus, 1758) and 142 of Abramis brama (Linnaeus,1758), collected from BeniHaroun dam (Mila, northeast Algeria). The fishes were subjected to some morphometric measurements and gill dissections and the harvested parasites were afterwards stored and identified. The parasitic indices were determined to examine the effect of size, sex, season and
A survey of Culicidae (Insecta Diptera) in some habitats in Souk-Ahras province (Northeast Algeria)
(2021-01-25) Hafs, N.,H; Hamaidia,K; Barour,Choukri; Soltani,N
A survey of immature Culicidae was conducted in diverse habitats of Souk-Ahras province (Northeast Algeria) between December 2018 and November 2019. Altogether, 12,861 specimens were collected and identified. Nineteen species of mosquitoes, belonging to two main subfamilies viz. Culicinae (accounts for 84.21 %) and Anophelinae (as 15.79 %), under 5 genera (Culex, Culiseta, Anopheles, Aedes and Uranotaenia). Among identified species, six were mentioned for the first time in the province of Souk-Ahras. The predominant species of the total mosquito fauna was Cx. pipiens L. with an abundance rate of 69.63 %. Two habitats were characterized by the highest prevalence of 11 species, Taoura (with 1 spp. Anophelinae, 10 spp. Culicinae) and Sedrata (with 11 spp. of Culicinae). However, Souk-Ahras site contained the largest abundance (41.20 %) belonging to 6 species. In addition, the spatial distribution of mosquitoes according to climatic factors (temperature, rainfall, humidity) was discussed. Data supported by some ecological indices of composition and structure revealed that the diversity level in Souk-Ahras province was between medium and very low compared to a scale varied between 0 and 1 (global Simpson index 0.50). The most diversified site in species was Taoura with the highest Shannon index value (2.11). Since vectors occurrence is constantly changing, it will be suggested that mosquito control should be intensified within other potential breeding sites in northeastern Algeria
ACUTE TOXICITY OF AN INSECTICIDE (ACETAMIPRID) ON LUMBRICUS TERRESTRIS (LINNAEUS, 1758
(2023-03-21) Berrouk, H; Necib, A; Hamaizia, Y; Chabi, C.,B; Hmaidia, K
Earthworms are effective bio-indicators of biodiversity, soil quality and the environmental impact on cropping systems. Therefore, this study was conducted to assess the toxicological effect of Acetamiprid, a widely used insecticide by farmers in the region of Souk-Ahras (Algeria), on a biological model named Lumbricus terrestris using seven increasing concentrations 0, 5, 7, 9, 12.5, 25 and 50 mg/500 mg soil for four control periods; 24, 48, 72 and 96 hours. The physicochemical soil analysis indicated that the soil pH was strongly alkaline, and the pH water and pH KCl were respectively 8.52 and 7.72. The soil texture was sandy loam with a high level of organic matter (OM = 12.9%). In addition, the LC50 and LC90 values were remarkably decreased (LC50 = 12.4, 10, 7.30 and 6.69/500 mg soil; LC90 = 18.52, 14.21, 9.50, 9.15 mg/500 mg soil, corresponding respectively for each LC values to the four exposure periods 24, 48, 72 and 96 hours). Furthermore, no significant change was noticed in body weight and growth of earthworms treated with Acetamiprid at various concentrations. The mortality rate was concentration-dependent; increased with increasing concentration, and this was supported by the histopathological observations showing cellular alterations in the epidermis and muscle fibers. Additionally, treatment with Acetamiprid at high concentrations caused marked morpho-logical abnormalities in the earthworms as evidenced by the winding of the body, loss of pigmentation, cut of the posterior part, lesions, or bloody wounds
Aeropalynological Analysis of Casuarina Genus (Casuarinaceae) in the Region of Sidi Amar (Annaba, North-East Algeria
(2014-12-22) Asma, N; Larbi, B
L’allergie au pollen, ou pollinose, est une affection saisonnière liée à la présence de grains de pollen des espèces anémophiles en abondance dans l’air. Dans la région méditerranéenne, elle affecte plus de 10 %de la population Afin d’étudier la teneur pollinique du Genre Casuarina (Casuarina-ceae ) dans l’atmosphère et l’effet des paramètres météoro logiques sur la production pollinique de ce genre, une étude aéropalynol ogique quotidienne au cours d’une période de 7 mois (Août 2012 - février 2013) a été réalisée dans la région de Sidi Amar (Sud d’Annaba) en utilisant la méthode gravimétrique de Durham (1946). Nos résultats montrent que le pollen de Casuarina est présent dans l'air à partir du début septembre jusqu'à la 1ère semaine de février. Les premiers grains apparaissent le 07/09/2012. Un total de 1956 grains de pollen (GP) a été capturé durant la période d'étude. L’émission pollinique est élevée au cours de la première semaine du mois de novembre (337 GP), la 2ème semaine de septembre (202 GP) et la 2ème semaine de décembre (167 GP). Alors qu'elle est restée nulle durant deux semaines au mois d’octobre. A partir des résultats statistiques, nous avons constaté qu’il existe une faible corrélation positive entre la production pollinique et la température moyenne, négative avec les précipitations et fortement négative avec la vitesse du vent.
Analyse de la variabilité morphométrique de Dactylogyrus heteromorphus El-Gharbi, 1994 (Monogenea : Dactylogyridae)
(2022-09-27) ALLALGUA, A; BOUCENNA, I; MENASRIA, A; BOUALLEG, C; BENSOUILAH, M; KAOUACHI, N
A study was carried out in the Ain Dalia dam to estimate the seasonal variability of the morphometric descriptors of Dactylogyrus heteromorphus,monogenean parasite of the teleost fish Luciobarbus callensis (Cyprinidae) during two seasons of the year 2021 (winter and spring). Sixteen morphometric variables of the body, attachment and copulatory organ were analysed to test the existence of differences between D. heteromorphus individuals in the winter and spring seasons. Significant differences (p<0.05) were found between the two seasons in five morphometric descriptors (body length, body width, total anchor length, distance between the sleeve and base of anchor and length of marginal hook). The other eleven variables measured (distance between handle and point, distance between guard and point, length of point, length of handle, length of guard, length of median bar, width of median bar, length of ventral bar, width of ventral bar, length of copulatory piece, width of copulatory piece) did not show any significant variation (p>0.05) between seasons
Anti-oxidant activity research of enzymatic biomarkers in protozoa (Paramecium sp.) under stress by manufactured nanoparticles
(2017-06-28) KHALDI, F; GRARA, N
The production of nanoparticles (NPs) is increasing rapidly for electronics, chemistry and biology applications. This interest is due to the very small size of NPs which provides them with many interesting properties such as rapid diffusion, high specific surface areas, reactivity in liquid or gas phase and a size close to bio-macromolecules. However, these extreme abilities might be a problem when considering a potentially uncontrolled exposure to the environment. For instance, nanoparticles might be highly mobile and rapidly transported in the environment or inside the body through a water or air pathway. Accordingly, the very fast development of these new synthetic nanomaterials raises questions about their impact on the environment and human health. We have studied the impact of manufactured nanoparticles (ZnO) on ciliated microorganisms (Paramecium sp.).Principles results show that the ZnO presence affects the paramecia growth. On the metabolic level, ZnO causes a disturbance in the rate of total protein and total carbohydrates. Regarding the biomarkers we identified disruption of glutathione (GSH)and catalase (CAT)activity. This study shows the importance of a model species for assessing the ZnO toxicity in laboratory tests. It is clear that ZnO is the oxidative stress source, which results in a stimulation of growth in Paramecium sp.,a disturbance in the total protein rate, carbohydrates, glutathione and catalase activity
Antibacterial Activity of Sulfonamide Derivatives against Clinical Strains of Bacteria
(2016-01-01) Bendjeddou, A; Abbaz, T; Khacha, N; Benahmed, M; Gouasmia,A; Villemin, D
This study was performed in order to evaluate antibacterial activity of some sulfonamides derivatives against four different clinical strains of bacteria namely, Proteus Mirabilis Pseudomonas Aeraginosa, Escherichia Coli and Staphylococcus Aureus. The zones of inhibition were performed with the disk diffusion method. Minimum inhibitory concentrations (MIC) of the compounds against the test microorganisms were determined by the dilution broth method. The result of the present study indicated that all the synthesized compounds exhibited a broad spectrum of activity against both gram-positive and gram-negative bacteria. Keywords: Sulfonamide; Antibacterial activity; Minimum inhibitory concentrations (MIC); disc diffusion technique
ANTIBACTERIAL ACTIVITY OF THE Artemisia herba-alba Asso ESSENTIAL OIL (SOUK AHRAS, ALGERIA) AGAINST FOURTEEN BACTERIAL STRAINS
(2019-09-30) BOUHOUIA, A; MAAZI, M.C; CHEFROUR, A
Artemisia herba-alba Asso (Asteraceae) essential oil from Fedj el dib region (Souk Ahras, Algeria) was obtained by hydro-distillation of aerial parts (stems, leaves and flowers) and analyzed by gas chromatography-mass spectrometry (GC/MS) to determine its chemical composition. The main compounds obtained are: 5,6-Dicarbadecaborane (68,33%), 1,5,5-Trimethyl-6- methylene-cyclohexene (7.00%), 4,8,12-Tetradecatrien-1-ol, 5,9,13-trimethyle (5.13%), Bicyclo-[3.1.1]heptane-2-methanol (4.39%) and Cyclo-pentane carboxylic acid 3-methylene (4.19%), Artemisia essential oil also contains Phenol, 2,3,5-trimethyl-(3.15%), Phosphoric acid tribornyl ester (0.61%) and beta.-Pinene (0.29%) and the Thujone (0.16%). Further to the analytic study of essential oil, a biological study was carried out; the purpose of this biological study was to assess the antibacterial properties of Artemisia herba-alba Asso essential oil against fourteen Gram-positive and Gram-negative bacterial strains using disk diffusion method. The results obtained reveal that the Artemisia herba alba Asso essential oil exerts a strong antimicrobial activity against all tested pathogenic bacteria except P. aeruginosa and Proteus vulgaris which were resistant even to the highest concentration of essential
Antioxidant and Anticholinesterase Effects of Methanol Extract, And Consecutive Fractions of Peganum harmala L.
(2023-06-01) Nait Marzoug A.,; Khaldi F.,; Ayari A.,; Gali L.,; Gheid A.
Peganum harmala L. is a medicinal plant growing in the region of Sidi Fredj of Souk Ahras city (Northeastern of Algeria), whose native populations commonly used this plant to treat various health conditions and illnesses. Up to now, no previous study on the beneficial effects of P.harmala in the Souk Ahras region has been conducted. Therefore, the present study aimed to investigate the phenolic and flavonoid composition, as well as the antioxidant activities of the methanol extract and its three fractions: chloroform, ethyl acetate and butanol from seeds of P. harmala using eight different methods. The inhibitory effect of the extracts against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) was also determined. The results showed that the ethyl acetate fraction contained the highest amounts of total phenolic and total flavonoid (237.23±1.06 µg GAE/mg and 82.58±0.03 µg QE/mg, respectively). Important antioxidant activity was exerted by the extracts, especially the ethyl acetate fraction, which was the most effective. However, a weak antioxidant activity in Ferrous ions chelation was observed for all extracts with >800 µg/mL. The samples showed also a strong inhibitory effect against AChE and BChE, the chloroform fraction was the most potent with IC50 values of 14.69±0.10 μg/mL against AChE and 7.36±0.77 μg/mL against BChE comparable to that of galantamine. Conclusively, the obtained results proved the beneficial antioxidant and anticholinesterase activities of P. harmala-derived bioactive molecules.
ASSESSMENT OF HEAVY METAL SOIL CONTAMINATION IN SOME NORTHEASTERN ALGERIAN BIOTOPES BY USING THE TERRESTRIAL SNAIL, HELIX ASPERSA
(2020-02-01) Guessasma, Z; Khaldi, F; Grara, N; Agouni, M; Sleimi, N.; Benslama MOHAMED
The study was aimed to assess the ecotoxicological impact of anthropogenic activities on soil quality by using the land snail Helix aspersa as a bioindicator. Soil samples and snails were collected from several sites of northeast Algeria during spring and winter, 2017. All sites were chosen in this study for the reason of their proximity to industrial factories as a potential source of heavy metal soil contamination. The concentration of heavy metals in soil samples was analyzed using the X-ray Fluorescence (XRF) spectrometer (Thermo Scientific Model Niton FXL 950) since the three metals of the highest levels in soil samples were examined in Helix aspersa hepatopancreas and feet by means of the atomic absorption spectrophotometry. Also, the highest levels of heavy metals were noticed during spring in Helix aspersa of the closest sites to the potential sources of pollution. These results are correlated with the physico-chemical characteristics of soil (texture, organic matter, pH Water, conductivity, limestone, and porosity) in each site
BIOCHEMICAL AND ENZYMATIC CHARACTERIZATION OF MACROPHYTE PLANT PHRAGMITES AUSTRALIS AFFECTED BY ZINC OXIDE
(2019-06-03) Khaldi, F; Menaiaia, K; Ouartane, N; Grara,N.
The research aimed to understand the behavior of aquatic plants subjected to xenobiotics, a macrophyte plant Phragmites australis from the region of Souk-Ahras, is treated with three concentrations based on zinc oxide (ZnO) (3, 6 and 12 nmol·mL-1) for 7, 14 and 21 days. A measurement of certain biochemical and enzymatic parameters characteristic of oxidative stress allowed us to evaluate not only the effect of zinc oxide but also the behavior of Phragmites australis subjected to this nanometric molecule. The statistical analysis of the results obtained showed no significant differences in all the parameters measured, between leaves and roots. The results of the analysis of heavy metals in wastewater, revealed values lower than the Algerian norm with the exception of Zn. On the other hand, we noticed a high concentration of Zn and a low concentration of Fe and Pb. This result makes it possible to conclude that the wastewater is characterized by a pollution of the metallic type loaded with partially degradable effluents.
Biochemical, quantitative and qualitative phenolic compounds, anti-free radical's activities of Calligonum comosum collected from different sites in the Algerian Desert
(2019-05-19) KHELEF, Y; CHOUIKH, A; REBIAI, A; NEFFAR,S; CHEFROUR, A; ADJAL, E.H; ALIA,F.
he aim of this study is to understand the effect on biochemical, quantitative and qualitative phenolic content and to estimate the anti-free radical's activities of the Calligonum comosum collected from various locations in Oued Souf region (Southeastern Algeria). Concerning the biochemical parameters, we registered pH values ranged between 4.8 and 4.2 and we observed oscillate in the amounts of proteins and the values of Catalase activity. The analysis of extracts by HPLC identified 43 compounds were counted as the maximum number of compounds, also we recorded the appearance of some phenolic compounds: Gallic acid, Chlorogenic acid, Vanillic acid, p-Comaric acid, Vanillin and Rutin in some extracts. Anti-free radical's activities which worked in two tests: DPPH● and TAC the results indicated that all extracts were highly anti-free radicals. Our results showed that there is a significant difference between the samples of Calligonum comosum collected in different sites
Biological parameters of Luciobarbus callensis populates Beni-Haroun dam, north-eastern Algeria
(2020-11-14) BERROUK, H; SAHTOUT, F; BOUALLEG, C.
Berrouk H, Sahtout F, Boualleg C. 2020. Biological parameters of Luciobarbus callensis populates Beni-Haroun dam, northeastern Algeria. Biodiversitas 21: 5691-5697. Fish age and growth data mainly assist many ichthyologists to understand the life history features of species and populations Growth is a major process of fish biology, and considered as one of the key processes of lengthstructured models of fishes. Thus, the present study was devoted to studying growth of an autochthonous species (Luciobarbus callensis), populating the dam of Beni-Haroun (Mila city, northeast of Algeria), and is known for its socio-economic value and aquaculture interest. The study was conducted on a sample of 257 fish individuals during the period 2015-2016. The age of L. callensis was determined by using the scalimetry method, providing easier sampling and reading procedures, and also high precision. Fish sexes were identified via the observation of gonads, by naked eye based on gonad shape and color, where the number of females was higher (165) than males (92).The obtained results revealed a maximum longevity of seven years of L. callensis, and the determined growth parameters of the separated and combined fish sexes were found to be as follow: L∞ = 47,3 cm; K = 0.29; t0 = -0.51; Ø’ = 2,813 (females); L∞ = 42,11 cm; K = 0.26; t0 = - 0.58; Ø’ = 2,663 (males); and L∞ = 47 37 cm; K = 0.20; t0 = -0.75; Ø’ = 2,652 (combined sex). Moreover, the evolution of the total weight of fishes with respect to their size presents minor allometry (b<3).(with or without sex distinction)
CADMIUM EXPOSURE ON Donax trunculus (MOLLUSCA BIVALVIA) LETHALITY PARAMETERS AND BIOMARKERS RESPONSES
(2022-01-11) Belabed, S; Soltani, N.
Cadmium is a non-essential heavy metal in the biological system. In this study, we have highlighted to evaluate of the effect toxicity of cadmium chloride (CdCl2) against Marine Bivalve Donax trunculus L. (Mollusca, Bivalvia) under laboratory conditions. The acute bioassay tests at various gradient concentrations of cadmium (5, 7.6, and 10 mg/L) were performed to estimate lethal concentrations 50 (LC50 Concentration estimated that produces a 50% mortality in a test population over a specific period) and 90 (LC90) for 24, 48, 72, and 96 hours of exposure. 96h-LC50 and 96h-LC90 values are: 2.59 and 9.25 mg/L respectively. In addition, the sublethal concentrations (LC10 and LC25) at acute exposure (96 h) which are: 0.72 mg/L and 1.32 mg/L respectively, and their fiducial limits were investigated. The results obtained indicate that cadmium causes mortality in the species studied with a Concentration-response relationship. Thus, specimens of this marine bivalve were experimentally exposed to CdCl2 at concentrations corresponding to 96h-LC10 and 96hLC50, to evaluate the effect of toxicity on catalase (CAT) and acetylcholinesterase (AChE) activities, followed by a recovery period during 4 days. Results showed that Cd inhibited AChE activity suggesting neurotoxicity disturbances. In addition, we observed a stimulation of the detoxification system as shown by an increase in catalase activity. Additionally, the transfer of individuals exposed to CdCl2 in untreated water showed a gradual restoration of the initial state. The data obtained from this study will provide useful information for understanding the toxicological process of CdCl2 in Donax trunculus.
Calcium Nitrate Toxicity on Rat Liver and Kidney Functions: A Biochemical and Histopathological Evaluation
(2022-06-30) Araar, S; Khaldi, F; Sayah, S; Chaib, S; Gheid, A.
Calcium NitrateTetrahydrate is a wide-used nitrogen fertilizer in Algerian agriculture. The present study was aimed to examine the toxic effects of calcium nitrate on kidney and liver functional biochemical markers. Twenty-eight male albino wistar male rats were divided into three treated groups receiving orally 200, 400 and 800mg/kg of calcium nitrate, and one untreated control group. Results showed a dose- dependent increase in kidney and liver relative weights, serum levels of glucose, cholesterol, triglyceride, blood urea, creatinine, and uric acid, and enzymatic activity of transaminases and alkaline phosphatase. However, serum protein and albumin levels were significantly decreased in a dose dependent manner as compared with those of control group. In addition, hepatic and renal histological changes were evidenced by hepatocyte degeneration, necrosis, dilation and sinusoid congestion, atrophy of glomeruli, vascular congestion, and infiltration of inflammatory cells. It is noteworthy that these adverse stress effects were higher in 400 and 800 mg/kg calcium nitrate treated rats than those treated with 200mg/kg and control group. In conclusion, the study proved the effective ability of subacute exposure of calcium nitrate to induce liver and kidney stress dysfunctions
Caracterisation Physico-Chimique Des Eaux Du Barrage Foum El-Khanga (Region De Souk-Ahras, Algerie)
(2017-01-01) Allalgua, A; Kaouachi, N; Boualeg, C; Ayari,A.
The objective of our work is to assess the quality of the dam Foum El-Khanga (east of Algéria) monthly during the year 2014. This study planned to make a physico-chemical characterization to highlight several parameters which come into play the determination of the quality of this water such as: temperature; pH; conductivity; turbidity; dissolved oxygen; title hydrotimetric; calcium; magnesium; title alcalimetric complet; nitrate; nitrite; ammoniacal nitrogen; sulfate; chloride; bicarbonate; phosphate; iron; biological oxygen demand (BOD) and chemical oxygen demands (COD). The results of physico-chemical analysis show that the water of this dam is characterized by a significant mineralization as indicated by high values of electrical conductivity and chloride. The results of principal component analysis (PCA) clearly showed a stronger relation between the hot season and temperature. However, the cold season was strongly correlated with mineralization and pollution parameters
CHEMICAL COMPOSITION AND ANTIBACTERIAL ACTIVITY OF ESSENTIAL OILS FROM Mentha pulegium L. AND Mentha suaveolens Ehrh. GROWING IN NORTH-EAST OF ALGERIA
(2020-09-09) BOUTABIA, L; TELAILIA, S; GUENADIL, F; CHEFROUR, A.
Mentha pulegium L. and M. suaveolens Ehrh. are spontaneous plants growing in the wetlands of the Souarekh region (north-eastern Algeria). They are very well known and used by the natives of this region for different virtues. Moreover, these subspecies have been chosen for their great morphological variations. Hence, in this study, we aim at determining the chemical composition of the essential oils of both plants and evaluating their antibacterial activities. The aerial parts of the plants were hydrodistilled using a Clevenger type apparatus and the essential oils were analyzed and identified using gas chromatography-mass spectrometry. Antibacterial screening of essential oils was carried out on the basis of the diameter of the inhibition zone using the disc diffusion method against two bacterial strains. The chemical analysis of the essential oils of both mints resulted in main compounds pulegone (61.24%), isomenthone (11.32%) and menthone (5.6%) for M. pulegium, and piperityone oxide (47.52%) for M. suaveolens. Accordingly, the study of the biological activity of essential oils manifested the antibacterial effect against the strains studied. The obtained results are therefore promising and thus pave the way for manufacturers to use the essential oils of the two plants in the pharmaceutical and food industries.
Chemical Composition and Antioxidant, Anti-Inflammatory, and Enzyme Inhibitory Activities of an Endemic Species from Southern Algeria: Warionia saharae
(2021-08-30) Rechek, H; Haouat, A; Hamaidia, K; Allal, H; Boudiar, T
Warionia saharae Benth. & Coss. (Asteraceae) is an endemic species of North Africa naturally grown in the southwest of the Algerian Sahara. In the present study, this species’ hydromethanolic leaf extract was investigated for its phenolic profile characterized by ultra-high-performance liquid chromatography coupled with a diode array detector and an electrospray mass spectrometer (UHPLC-DAD-ESI/MS). Additionally, the chemical composition of W. saharae was analyzed by gas chromatography–mass spectrometry, and its antioxidant potential was assessed through five in vitro tests: DPPH● scavenging activity, ABTS●+ scavenging assay, galvinoxyl scavenging activity, ferric reducing power (FRP), and cupric reducing antioxidant capacity. The UHPLC-DAD-ESI/MS analysis allowed the detection and quantification of 22 compounds, with taxifolin as the dominant compound. The GC–MS analysis allowed the identification of 37 compounds, and the antioxidant activity data indicate that W. saharae extract has a very high capacity to capture radicals due to its richness in compounds with antioxidant capacity. The extract also showed potent α-glucosidase inhibition as well as a good anti-inflammatory activity. However, weak anti-α-amylase and anticholinesterase activities were recorded. Moreover, an in silico docking study was performed to highlight possible interactions between three significant compounds identified in W. saharae extract and α-glucosidase enzyme.
COMPARATIVE STUDY BETWEEN THE TOXICITY OF ALUMINIUM CHLORIDE AND MERCURIC CHLORIDE ON BIOCHEMICAL MARKERS AND LIPID PROFILES IN WISTAR RATS
(2022-06-07) Sayah, S; Khaldi, F; Araar, S; Chaib, S; Gheid, A.
This study was designed to compare the between the toxic of aluminum chloride (AlCl3) and mercuric chloride (HgCl2) on biochemical markers of liver function, and lipid profiles in Wistar rats. Forty-two male albino Wistar rats were equally divided into three main groups as untreated control (n= 6) group and two treated (n = 6) groups which then were subdivided each into three different subgroups depending on the metal type and doses, namely AlCl3 at doses 7.6, 12.66, and 38 mg/kg body weight (bw), and HgCl2 at doses 0.1, 0.2, and 0.4 mg/kg (bw). Treatments were given to rats orally for 28 days. Results showed a decrease in body weight, and an increase in relative and absolute liver weights, in addition to a significant increase in the levels of serum glucose, bilirubin (total and direct), cholesterol and triglycerides, and the enzymatic activities of transaminases (TGO, TGP) and alkaline phosphatase (PAL), and conversely a decreased level of total proteins in Al and Hg treated animals compared to the controls. The biochemical alterations were supported by the histopathological evaluation of the liver, showing vascular congestion, sinusoid disintegration, centrilobular vein disintegration, and inflammatory cell infiltration. In conclusion, AlCl3 and HgCl2 have induced liver dysfunction, and HgCl2 was proved to be a very toxic meta