Browsing by Author "Barour, C"

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    Diatoms diversity and dynamics in a southern Mediterranean lagoon (Mellah, Algeria)
    (2019-06-18) Draredja, M. A; Barour, C; Frihi, H; Boualleg, C; Laabir, M.
    The composition and distribution of diatoms were studied using a biweekly sampling performed in three representative stations in the Mellah Lagoon (South Western Mediterranean, Algeria), during 2016. In total, 160 diatoms species - belonging to centric species (52 species) and pennate species (108 species) - were inventoried. The relative abundance of the pennate forms (67.50%) is clearly higher compared to the centric forms (32.50%). The blooms of diatoms are represented by 3 species: Chaetoceros constrictus Gran, 1897 with up to 15000 cells.L-1 (end of May), Chaetoceros sp. with up to 178320 cells.L-1 (early October), and Nitzschia longissima (Brébisson) Ralfs, 1861 with up to 200640 cells.L-1 (early September). Proliferations of three potentially toxic species: Pseudo-nitzschia group delicatissima (Cleve) Heiden, 1928 (2500 cells.L-1), Pseudo-nitzschia group seriata (Cleve) H. Peragallo, 1899 (700 cells.L-1) and Thalassionema nitzschoides (Grunow) Mereschkowsky, 1902 (210 cells.L-1), requires regular monitoring of this ecosystem where the exploitation of the shells is practiced. The diatom populations seem to be structured showing diversity (H’) and equitability (J’) indices reaching 4.56 bits.cell and 0.98 respectively, with the exception of springsummer and summer-autumn transition periods
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    Evaluation of solvent influence on phytochemical content and antioxidant activities of two Algerian endemic taxa: Stachys marrubiifolia Viv. and Lamium flexuosum Ten. (Lamiaceae)
    (2020-12-10) Bouasla, I; Hamel, T; Barour, C; Bouasla, A; Hachouf, M; Bouguerra, O.M; Messarah, M
    Introduction: Stachys and Lamium genera have been widely used in traditional medicine. This is the first study attempting to determine the biological active compounds, mineral elements content and in vitro antioxidant activities of two Algerian endemic taxa (Stachys marrubiifolia Viv. and Lamium flexuosum Ten.), as well as evaluating the solvent influence on our studied parameters. Methods: This experimental study conducted phytochemical screening, followed by mineral elements content determination. Extracts were prepared using various solvents with different polarities (water/methanol, ethyl acetate, and butanol), after that polyphenols, flavonoids, tannins, flavanols, and orthodiphenols levels were determined. In addition, the in vitro radical scavenging evaluation (DPPH, NBT/Riboflavin and FRAP assay) and the total antioxidant properties (훽-carotene/linoleic acid and phosphomolybdenum assay) were assessed. Results: The obtained results clearly showed mineral elements richness and demonstrated that different extracts contained various biological active compounds with substantial amounts (high content of flavonoids, medium content of orthodiphenols, anthocyanins as well as flavanols, and low content of total phenols as well as tannins). Ethyl acetate was shown to be the most effective for the extraction of these compounds. Also, we noted that all extracts had diverse antioxidant capacities with IC50 values varying between (0.18±0.05 and 3.12±0.18 mg/g), and the relative differences depended on species, fractions, and testing methods. Conclusion: Our study clearly shows that solvent extracts had a remarkable influence on all studied parameters, confirming the strong correlation between phytochemical constituents and antioxidant activities. Therefore, it justifies the use of these endemic taxa in traditional medicine.
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    Multi-biomarkers approach to the assessment of the southeastern Mediterranean Sea health status: Preliminary study on Stramonita haemastoma used as a bioindicator for metal contamination
    (2018-05-25) Bouzahouane, H; Barour, C; Sleimi, N; Ouali, K.
    The present study aimed to evaluate the responses of different biochemicals parameters associated with environmental pollution in the digestive gland of the gastropod mollusc Stramonita haemastoma. Physiochemical parameters and trace metal elements (Copper (Cu), Zinc (Zn), Chromium (Cr), Cadmium (Cd) and Lead (Pb)) were measured in seawater. Spatiotemporal variations in reduced glutathione (GSH), malondialdehyde (MDA) and metallothionein (Mt) as well as the specific activities of glutathione Stransferase (GST) and catalase (CAT) were evaluated in digestive gland of this species during a one-year period in 2013e2014. Samples collection was conducted at three sites. The results obtained showed seasonal fluctuations in GST and CAT activities and in the rate of Mt content. In addition, intersite variations in GSH, MDA, Mt and CAT were recorded in individuals. Also, trace metal elements concentrations determined by season in the digestive gland revealed spatial and temporal variations for Cu and Zn but they are below the limit of detection for Cd and Pb. The highest values were generally recorded in spring for Cu and in winter for Zn. In this first regional study using in S. haemastoma as a model, the biomarkers measured were seen to be inducible parameters to evaluate the health state of the organism and the overall quality of the study sites