Browsing by Author "Hameurlaine Samir"

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Assessment of Alkaloid Content and Antibacterial Activity of Hyoscyamus Albus and Hyoscyamus Muticus Collected in Two Different Climatic Regions in Algeria
(SEVAS EDUCATIONAL SOC, 2019-03-15) Kebaili Zoubir; Hameurlaine Samir; Fellah Ouroud; Djermane Mohamed; Gherraf Noureddine; Zellagui Amar; Abidi Abdenabi; Derouiche Kamel
Medicinal plants are considered as a very important source of secondary metabolites and therefore bioactive molecules. Our comparative study deals with two species: Hyoscyamus albus and Hyoscyamus muticus harvested in two different climate regions namely: Arris (semi-arid, wilaya of Batna) and Djanet (arid, wilaya of Illizi), respectively. The phytochemical screening revealed that aerial parts of the two species contain almost all classes of secondary metabolites such as flavonoids, quinones, anthraquinones, saponins, tannins, coumarins and alkaloids. The quantitative assessment of alkaloids in hydroalcoholic extracts of the two plants showed that the total alkaloid content varies depending upon the species and climate conditions. The alkaloid level was found to be 0.51% and 0.32% in Hyoscyamus albus and Hyoscyamus muticus respectively. Moreover, the antibacterial test vis-à-vis some pathogen bacteria such as: Escherichia coli, Staphylococcus aureus, Staphylococcus aureus and Pseudomonas aeruginosa showed that both alkaloid crude extracts and hydroalcoholic extracts are moderately active against the tested bacterial strains.
Editor's choice Water Extract of Tamarix gallica L. as effective Agents against C6 and HeLa tumor cell lines
(De Gruyter Poland Bogumiła Zuga 32A Str. 01-811 Warsaw, Poland, 2019) Fellah Ouroud 1, 2, 3,; Hameurlaine Samir; Djermane Mohamed; Tahar Wafa; et al
The aerial parts of Tamarix gallica L. taken from three different climatic stages in Algeria (Oum El Bouaghi: Semi-arid, ElTaref: Humid, and Ouargla: Arid) were extracted using boiling distilled water. The crude extracts were subjected to total phenolics and flavonoids quantifications in addition to anti-proliferative assessment against two tumor cell lines namely rat brain tumor (C6) and human cervix carcinoma (HeLa) using BrdU (bromo-deoxyuridine) ELISA(Enzyme-linked immune-sorbent assay) and xCELLigence assay. The total phenolics yield was found to range between 16.14 and 39.32 mg GAE (Gallic acid equivalent)/g of extract and a flavonoids yield ranging between 16.51 and 20.35 mg QE(quercetin equivalent)/g of extract. The various phenolics were identified using HPLC-TOF/MS to highlight hesperidin and rosmarinic Acid as major components. Moreover, the extracts exhibited different levels of antitumor potency against C6 and HeLa cell lines depending upon the climatic stage and the concentration. A good cytotoxic effect was recorded with the species collected from the humid region at 250 µg/mL. On the contrary, the other extracts revealed a weak activity for both tests.