Department of Process Engineering
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Item Assessment of Alkaloid Content and Antibacterial Activity of Hyoscyamus Albus and Hyoscyamus Muticus Collected in Two Different Climatic Regions in Algeria(SEVAS EDUCATIONAL SOC, 2019-03-15) Kebaili Zoubir; Hameurlaine Samir; Fellah Ouroud; Djermane Mohamed; Gherraf Noureddine; Zellagui Amar; Abidi Abdenabi; Derouiche KamelMedicinal plants are considered as a very important source of secondary metabolites and therefore bioactive molecules. Our comparative study deals with two species: Hyoscyamus albus and Hyoscyamus muticus harvested in two different climate regions namely: Arris (semi-arid, wilaya of Batna) and Djanet (arid, wilaya of Illizi), respectively. The phytochemical screening revealed that aerial parts of the two species contain almost all classes of secondary metabolites such as flavonoids, quinones, anthraquinones, saponins, tannins, coumarins and alkaloids. The quantitative assessment of alkaloids in hydroalcoholic extracts of the two plants showed that the total alkaloid content varies depending upon the species and climate conditions. The alkaloid level was found to be 0.51% and 0.32% in Hyoscyamus albus and Hyoscyamus muticus respectively. Moreover, the antibacterial test vis-à-vis some pathogen bacteria such as: Escherichia coli, Staphylococcus aureus, Staphylococcus aureus and Pseudomonas aeruginosa showed that both alkaloid crude extracts and hydroalcoholic extracts are moderately active against the tested bacterial strains.Item Eco-Extraction of Essential Oil of the Species Salvia Officinalis L. Systematic Characterization and Parametric Modeling(EURESIAN PUBLICATIONS, 2018-07-23) DJERMANE Mohamed; Abidi Abdenabi; Cheraif Imed; Toubal Abdelaziz; Noureddine GherrafIn this study, the response surface methodology (RSM) was used to optimize the hydrodistillation process of Algerian Salvia officinalis essential oil. The effects of grain size, condensing flow rate and extraction time on oil yield were studied. A 3-factor design by the BoxBehnken method was used to produce factor combinations, from the response surface analysis. A second-order polynomial expression was deduced and used to determine the best yield efficiency of extracted oil according to the optimal conditions. Analysis of variance (ANOVA) indicates that the generated second-order polynomial model was highly significant with R2 = 0.9589 and P <0.006. Optimum oil yield conditions (0.98%) were estimated at a particle diameter of 2mm, a condensation flow rate of 1.4mL/min of and an extraction time of 210min. The essential oil extracted from the sage leaves by hydrodistillation was analyzed by GC/MS, to afford α.-Thujone, camphor, 1,8- cineole and β.-Thujone as main components.