Faculty of Natural Sciences and Life

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Author: search.filters.author. Necib, AStart date: 1640

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    Physicochemical and Melissopalynological Study of Some Honey Samples from the Algerian East Region
    (2023-03-20) Ketfi, L; Draiaia, R; Necib, A; Mohamadi, N
    The quantitative and qualitative analysis of honey components is the object of this study. These analyses are considered a physicochemical and melissopalynological tool that allows the study of some characteristics of honey. The analysis of ten (10) samples of Algerian honey is carried out to determine the pH, water content, electrical conductivity, ash content, acidity, hydroxymethylfurfural content, proteins, and sugar content. According to the obtained results, the physico-chemical parameters of the studied honeys comply with the European and international standards. The pollen spectrum indicated the presence of 23 plant families, mainly Fabaceae, encompassing a total of 65 honey species visited by foragers. Of the 10 honeys sampled and analyzed, six were monofloral, with the remainder showing no apparent dominance of any honey type. The most frequent plant species were eucalyptus (Eucalyptus sp.), Italian sainfoin (Hedysarum coronarium), and chickweed (Lathyrus sp.). Furthermore, the results obtained indicate that all honey (monofloral and polyfloral) showed a high degree of variability in the number of pollen grains
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    ACUTE TOXICITY OF AN INSECTICIDE (ACETAMIPRID) ON LUMBRICUS TERRESTRIS (LINNAEUS, 1758
    (2023-03-21) Berrouk, H; Necib, A; Hamaizia, Y; Chabi, C.,B; Hmaidia, K
    Earthworms are effective bio-indicators of biodiversity, soil quality and the environmental impact on cropping systems. Therefore, this study was conducted to assess the toxicological effect of Acetamiprid, a widely used insecticide by farmers in the region of Souk-Ahras (Algeria), on a biological model named Lumbricus terrestris using seven increasing concentrations 0, 5, 7, 9, 12.5, 25 and 50 mg/500 mg soil for four control periods; 24, 48, 72 and 96 hours. The physicochemical soil analysis indicated that the soil pH was strongly alkaline, and the pH water and pH KCl were respectively 8.52 and 7.72. The soil texture was sandy loam with a high level of organic matter (OM = 12.9%). In addition, the LC50 and LC90 values were remarkably decreased (LC50 = 12.4, 10, 7.30 and 6.69/500 mg soil; LC90 = 18.52, 14.21, 9.50, 9.15 mg/500 mg soil, corresponding respectively for each LC values to the four exposure periods 24, 48, 72 and 96 hours). Furthermore, no significant change was noticed in body weight and growth of earthworms treated with Acetamiprid at various concentrations. The mortality rate was concentration-dependent; increased with increasing concentration, and this was supported by the histopathological observations showing cellular alterations in the epidermis and muscle fibers. Additionally, treatment with Acetamiprid at high concentrations caused marked morpho-logical abnormalities in the earthworms as evidenced by the winding of the body, loss of pigmentation, cut of the posterior part, lesions, or bloody wounds
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    Investigation and quantification of the potential antioxidant, inflammatory, and antibacterial bioactive molecules of the extracts of Algerian black and green table olive brine
    (2023-01-12) Mohamadi,N; Meraghni, M; Meradci, F; Necib, A; El Arbi, M; Elhadef, K; Smaoui, S; Bouaziz, M.
    table olive industry produces a large amount of wastewater that can be expensive to be treated and harmful to the environment. This study aimed to find a way to reuse brine water from the production of black and green table olive brines from Bejaia and Mascara of the Sigoise cultivar in order to create a valuable byproduct and contribute to sustainable development. In this context, the high-performance liquid chromatography–diode-array detection (HPLC-DAD) analysis revealed the highest concentration of hydroxytyrosol (4-(2-dihydroxy phenyl ethanol); 69.67 mg/100 mg) for green table olive brines of Mascara (EOGM) and tyrosol (Ty) (28.8 mg/100 mg) for black table olive brines of Bejaia (EOBB). Presence of polyphenols and ortho-diphenols could be responsible for their antioxidant, anti-inflammatory, and antibacterial properties. To assess antioxidant activity, the scavenging effects of DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,20-Azinobis[3-ethylbenzothiazoline-6-sulfonate]) radicals as well as hydrogen peroxide (H2O2) radicals were used. The antimicrobial activity showed that the black olive extract exhibited the best inhibitory effect, with a minimum inhibitory concentration (MIC) ranging from 0.625 mg/mL to 0.31 mg/mL. The anti-inflammatory activity of tested extracts of black olives of Bejaia (EOBB) and green of olives Mascara (EOGM) was 20.06 μg/mL and 20.21 μg/mL, respectively, which demonstrated the anti-inflammatory effect of these extracts on human beings.
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    La bio-indication de la pollution aquatique par les microalgues (Cas de l'Oued "Bounamoussa" et du Lac des" Oiseaux"
    (2013-01-30) Necib, A; Rezig, H; Boughedir, L.
    Continental aquatic environments are complex, fragile and governed by specific physico- chemical ecosystems laws. Microalgae that colonize living precariously and totally dependent. The most tolerant genera adapt to the input of an exogenous substance or a change in physico-chemical parameters. We used microalgae as biological indicators of water pollution in two sites: Bounamoussa's River and Lake of the Birds (W.Tarf). The method applied is the index of organic pollution recommended by Palmer (1969), which is to translate the level of contamination in water samples. The observation of morphological characteristics of collected microalgae allowed us to identify 20 genera. We recorded 10 genera in Bounamoussa's River: Microcystis, Euglena, Lepocinclis, Gomphonema, Melosira, Navicula, Synedra, Chlorella, Closterium and Scenedesmus. At the Lake of the Birds, we noted the existence of 17 genera: Aphanizomenon, Anabaena, Cylindrospermum, Merismopedia, Microcystis, Phormidium, Synechocystis, Phacus, Cyclotella, Gomphonema, Navicula, Synedra, Ankistrodesmus, Chlorella, Closterium, Pediastrum and Scenedesmus. The results show that the highest algal densities are recorded in the Lake of the Birds, followed by the Bounamoussa's River. According to the pollution index Palmer (1969), we found that there is little organic pollution (index = 9) at Bounamoussa's River. However, the contamination at the site of the Lake of the Birds by organic matter is considered average (index= 12). Keywords: Organic pollution -Microalgae - Bioindication -Pollution tolerant genera of algae -Palmer index (1969).
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    Evaluation of Polyphenols, Vitamin C Contents and Antioxidant Activity of Two Types of Algerian Honey
    (2022-09-28) Necib, A; Ketfi, L; Draiaia, R; Rezig, S.
    This study aimed to estimate two different types of Algerian honey: antioxidant contents (total phenols and vitamin C), and levels of antioxidant (DPPH assay) and reducing activity (FRAP assay). Honey samples were characterized by high content of total phenols (314.231 ± 281.346 – 394.231 ± 155.835 mg AG 100 g-1) and low vitamin C values (0.25 ± 0.05 – 0.35 ± 0.05 mg 100 g-1). As a result, the antioxidant activity and reducing capacity values were found to be 9.578 ± 3.157 and 11.255 ± 2.668% for DPPH and 15.240 ± 4.578 to 17.794 ± 8.179 μg 100 g-1 for the FRAP assay. Our data showed that dark honey contains bioactive compounds with significant antioxidant activity