Department of Material Sciences

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Author: search.filters.author.Abdelhak GheidStart date: 2020

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    Effect of Peganum harmala L. extract supplemented ISP2 medium on growth and production of secondary metabolites of Streptomyces ayarius S115
    (Elsevier, 2022-12-22) Amel Nait Marzoug; Adel Ayari; Fadila Khaldi; Ines Guehria; Abdelhak Gheid
    Background: The present work aimed to investigate the effect of Peganum harmala L. in increasing the growth and production of secondary metabolites of Streptomyces ayarius strain S115 via ISP2 culture medium. Peganum harmala L. was dried and added to ISP2 medium. The morphological properties and the antibacterial activity of S. ayarius strain S115 seeded in ISP2 and the modified ISP2 media was evaluated by using the agar well diffusion method against five pathogenic bacteria, Escherichia coli Klebsiella pneumoniae, Pseudomonas aeruginosa, Serratia sp. and Methicillin-Resistant Staphylococcus aureus. The biomass of S. ayarius strain S115 in both media was determined. The kinetics of growth and production of secondary metabolites were studied for 7 d in different carbon sources. Results: Culture on the modified ISP2 showed an effective growth of S. ayarius strain S115 with changed color of the aerial mycelium from gray to white. The antibacterial activity revealed large inhibition zones against the tested pathogenic bacteria compared to those of the ISP2. The amount of S. ayarius strain S115 biomass was twice as high in the modified ISP2. The effect of different carbon sources on the growth and production of secondary metabolites of S. ayarius strain S115 revealed the highest biomass and biological activities through using glucose in the modified ISP2 on the 3rd day of culture. Of note, glycerol was found as the optimal carbon source in ISP2.
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    Groundwater Quality Evaluation (Four boreholes from the region of Tebessa - Algeria)
    (Annals of R.S.C.B., 2021-12-15) Khedidja Touahria; Yamina Berredjem; Fethi Baali; Soraya Touahria; Samoun Fatah; Abdelhak Gheid
    Tebessa is located in the north-eastern of Algeria. It has a semi-arid climate with a water balance deficit. Groundwater is a significant source for drinking water supply and irrigation of agricultural land in many parts of this area. The water quality depends on the geological influence and anthropogenic pollution. Continuously increasing abstraction of groundwater resources to meet rising agricultural and domestic needs leads to a growing deficit of water. The intensive exploitation of the available water resources, coupled with periods of drought, has led to lowering of the water table and increasing the risk of degradation of water quality, especially when abstraction amounts greatly exceed the natural recharge of aquifers. The study of the physicochemical water quality of four boreholes of Tebessa shows that the waters studied are of the same hydro-chemical facies (calcium sulphate). The results of the physicochemical analyses are compared with national and international standards. In addition The Groundwater Quality Evaluation System (Groundwater - QES) is used to classify the waters studied depending on the quality and suitability for the production of drinking water.