Department of Material Sciences

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    Effect of Peganum harmala L. extract supplemented ISP2 medium on growth and production of secondary metabolites of Streptomyces ayarius S115
    (Elsevier, 2022-12-22) Amel Nait Marzoug; Adel Ayari; Fadila Khaldi; Ines Guehria; Abdelhak Gheid
    Background: The present work aimed to investigate the effect of Peganum harmala L. in increasing the growth and production of secondary metabolites of Streptomyces ayarius strain S115 via ISP2 culture medium. Peganum harmala L. was dried and added to ISP2 medium. The morphological properties and the antibacterial activity of S. ayarius strain S115 seeded in ISP2 and the modified ISP2 media was evaluated by using the agar well diffusion method against five pathogenic bacteria, Escherichia coli Klebsiella pneumoniae, Pseudomonas aeruginosa, Serratia sp. and Methicillin-Resistant Staphylococcus aureus. The biomass of S. ayarius strain S115 in both media was determined. The kinetics of growth and production of secondary metabolites were studied for 7 d in different carbon sources. Results: Culture on the modified ISP2 showed an effective growth of S. ayarius strain S115 with changed color of the aerial mycelium from gray to white. The antibacterial activity revealed large inhibition zones against the tested pathogenic bacteria compared to those of the ISP2. The amount of S. ayarius strain S115 biomass was twice as high in the modified ISP2. The effect of different carbon sources on the growth and production of secondary metabolites of S. ayarius strain S115 revealed the highest biomass and biological activities through using glucose in the modified ISP2 on the 3rd day of culture. Of note, glycerol was found as the optimal carbon source in ISP2.
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    Removal of methylene blue from water using eggshell membrane fixed bed
    (Desalination and Water Treatment, 2017-01-16) Radia Zerdoum; Zhour Hattab; Yamina Berredjem; Radia Mazouz; Ridha Djellabi; Naima Filali; Abdelhak Gheid; Kamel Guerfi
    The purpose of this work was to remove methylene blue (MB) from water using natural eggshell membrane (ESM) in fixed-bed column. The ESM was treated and characterized by Fourier transform infrared spectra (FTIR), scanning electron microscope (SEM) and X-ray diffraction (XRD). A series of experiments were carried out to investigate the effects of bed height on breakthrough curve, flow rate, MB concentration, solution pH (2–10), the ionic strength and temperature on the adsorption behavior. The experimental results show that the adsorption capacity increases with the increase in the bed depth and MB concentration. However, it decreases with increasing the flow rate and temperature. The ESM adsorption capacity of MB is more pronounced in basic medium. The addition of NaCl salt ions decreases the fixation of MB on ESM. Five kinetic models, Bohart–Adams, Wolborska, Thomas, Yoon–Nelson and bed depth service time (BDST) models were applied to experimental data in order to predict the breakthrough curves using non-linear regression and to determine the characteristic parameters of the column useful for process design. The results showed that Thomas and Yoon–Nelson models were found suitable for the normal description of breakthrough curve at the experimental condition, while Adams–Bohart and Wolborska models were only for an initial part of dynamic behavior of the ESM column. Desorption of MB from ESM bed was performed with distilled water as the desorbing agent, and reuse study was investigated.
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    Utilization of Powdered Eggshell Waste for Rhodamine B Removal: Evaluation of Adsorptive Efficiencies and Modeling Studies
    (American Scientific Publishers, 2018-02-03) Wahiba Bessashia; Zhour Hattab; Yamina Berredjem; Ridha Djellabi; Radia Zerdoum; Assia Allaoui; Abdelhak Gheid; Kamel Guerfi
    Biomass wastes are being recognized as emerging functional platforms that can be utilized in various fields. Eggshell, which can be found everywhere due to the large eggs consumption, has usually been regarded as waste and overlooked. In this contribution, we report the use of three powders prepared from eggshell waste such as Eggshell without membrane (ES), Eggshell membrane (ESM) and Eggshell with membrane (ESM-ES) as biosorbents for the removal of Rhodamine B (RhB) from water. Biosorbent powders were characterized by XRD, SEM, BET and FTIR. The effects of initial RhB concentration, temperature and pH on dye adsorption were performed. The results showed that the ESM, due to its 3D network porous structure, exhibited the highest adsorption efficiency than ES and ES-ESM. The RhB sorption on these adsorbents obeys pseudo-second-order kinetics which indicates a chemisorption process. For ESM adsorbent, the Freundlich isotherm model has a best-fit compared to Langmuir and Temkin isotherm models (multilayer dye adsorption with non-uniform distribution). However, for ES and ESM-ES adsorbents Langmuir has a best-fit than the other models (monolayer dye adsorption).
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    Groundwater Quality Evaluation (Four boreholes from the region of Tebessa - Algeria)
    (Annals of R.S.C.B., 2021-12-15) Khedidja Touahria; Yamina Berredjem; Fethi Baali; Soraya Touahria; Samoun Fatah; Abdelhak Gheid
    Tebessa is located in the north-eastern of Algeria. It has a semi-arid climate with a water balance deficit. Groundwater is a significant source for drinking water supply and irrigation of agricultural land in many parts of this area. The water quality depends on the geological influence and anthropogenic pollution. Continuously increasing abstraction of groundwater resources to meet rising agricultural and domestic needs leads to a growing deficit of water. The intensive exploitation of the available water resources, coupled with periods of drought, has led to lowering of the water table and increasing the risk of degradation of water quality, especially when abstraction amounts greatly exceed the natural recharge of aquifers. The study of the physicochemical water quality of four boreholes of Tebessa shows that the waters studied are of the same hydro-chemical facies (calcium sulphate). The results of the physicochemical analyses are compared with national and international standards. In addition The Groundwater Quality Evaluation System (Groundwater - QES) is used to classify the waters studied depending on the quality and suitability for the production of drinking water.
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    Experimental and numerical study on the leaching of pesticides into the groundwater through a porous medium: Effects of transport parameters
    (2019-01-06) Khoula Haddad; Abdelhak Gheid; Djamel Haddad; Kafia Oulmi
    This study aims to investigate, experimentally and numerically, the pesticide transport through the soil in unsaturated zone. The movement of pesticide is described with one dimensional model. The transport equation incorporates terms accounting for diffusion –convection, adsorption and reaction. The first objective of this work was to identifies the effects of the parameters transport such us; the dimensions first order degradation coefficient (µ), the peclet number (ρe) and the dimensionless mass transfer coefficient (w). The evolution of pesticide concentration in function of depth and time was found by using the non-equilibrium transport model. The data obtained show the importance of the adsorbed concentration. The second objective is to find the relation between the concentration and the transport parameters because there is a difficulty of the analytical solution. The solution of the problem was carried out by a numerical approach by using the finite difference method and a Fortran program.