Browsing by Author "Grara, N"

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    ASSESSMENT OF HEAVY METAL SOIL CONTAMINATION IN SOME NORTHEASTERN ALGERIAN BIOTOPES BY USING THE TERRESTRIAL SNAIL, HELIX ASPERSA
    (2020-02-01) Guessasma, Z; Khaldi, F; Grara, N; Agouni, M; Sleimi, N.; Benslama MOHAMED
    The study was aimed to assess the ecotoxicological impact of anthropogenic activities on soil quality by using the land snail Helix aspersa as a bioindicator. Soil samples and snails were collected from several sites of northeast Algeria during spring and winter, 2017. All sites were chosen in this study for the reason of their proximity to industrial factories as a potential source of heavy metal soil contamination. The concentration of heavy metals in soil samples was analyzed using the X-ray Fluorescence (XRF) spectrometer (Thermo Scientific Model Niton FXL 950) since the three metals of the highest levels in soil samples were examined in Helix aspersa hepatopancreas and feet by means of the atomic absorption spectrophotometry. Also, the highest levels of heavy metals were noticed during spring in Helix aspersa of the closest sites to the potential sources of pollution. These results are correlated with the physico-chemical characteristics of soil (texture, organic matter, pH Water, conductivity, limestone, and porosity) in each site
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    Phytochemical Analysis, Antioxidant, and Acetylcholinesterase Inhibitory Activity of Propolis from Northeastern Algeria
    (2022-01-01) Ouahab, A; Grara, N; Menaiaia, K; Khaldi, F; Bensouic, Ci
    Propolis is a bee resinous substance consisting mainly of phenolic compounds having nutritional and therapeutic properties and formed by the mixing of the tree and plant secretions collected by honey bees. Herein, the present study was aimed to assess the antioxidant and anticholinesterase activity of ethanolic and acetone propolis extracts from two sampling sites in Souk Ahras city (northeast Algeria). The antioxidant activity of the extracts was determined by using the common antioxidant assays (1,1- diphenyl-2-picrylhydrazyl [DPPH], acide 2-2’-azino-bis (3- ethylbenzothiazoline-6-sulphonique) [ABTS], galvinoxyl radical [GOR], and cupric reducing antioxidant capacity [CUPRAC]), and the anticholinesterase activity was determined against acetylcholinesterase (AChE). Moreover, the total phenolic (TPC), flavonoid (TFC), and condensed tannins (CTC) contents were quantified. The propolis extracts showed a potent antioxidant/inhibitory activity which almost met that of synthetic antioxidants used as standards (butylated hydroxytoluene [BHT] and butylated hydroxyanisole [BHA]). In addition, the AChE activity was highly strongly inhibited in a dose-dependent manner by the propolis extracts. The propolis extracts proved their richness in bioactive molecules able to enhance various biological activities
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    THE USE OF MACROPHYTE TYPHA LATIFOLIA FILTERS IN THE TREATMENT OF WASTEWATERS OF MEDJERDA RIVER, IN SOUK-AHRAS CITY (NORTH-EAST ALGERIA)
    (2019-01-01) Mamine, N; Grara, N; Khaldi, F.
    The present study was aimed to test the purifier capacities of a helophyte plant, and herein the cattail (Typha latifolia) has been used to purify the raw wastewaters through monitoring the contamination physico-chemical and parasitological indicators following various stay periods. The experimental pilot scheme is composed of three planted trays of young Typha stem. Each one was retained, respectively a treatment period of 7, 14 and 21 days. The supply of planted systems are exclusively occurred through the release of wastewaters in Medjerda river (discharge of river).The results have shown that these helophytes lead to the neutralization of pH, along with decrease in electrical conductivity of raw wastewaters. The monitoring of the purifying performances of the three systems shows that the third planted tray ensure an important elimination of the total suspended matter (TSS) at 97.6 % and the turbidity (92.4 %), which showed to be efficient in removing the organic charge with a reduction in BOD5 (90.1 %), COD (92.66 %) and the nutrients (NH4+: 88.54 % and PO43-: 91.9 %). Moreover, the concentrations of these chemicals do not exceed the national (J.O.R.A) and international (WHO, FAO) standards for the irrigation water. Regarding the parasite load, the three planted trays lead to the elimination of the total parasitic helminth eggs
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    Toxicité subChronique des Nanoparticule de ZnO chez Helix aspersa: Etude physiologique et Comportementale
    (2017-10-10) Grara, N; Khaldi, F; Benamara, A; Zenir, Z; Abdemadjid, S; Bouloudenine, M
    In this study we were interested in assessing the impact of ZnO based metal nanoparticles and their effects on body bioaccumulative bioindicator of environmental pollution and an indicator of health status Helix aspersa for the preservation of Human health. This is a sub chronic (28 days) by means of digestive absorption. The toxicity of ZnO is determined in the snail Helix aspersa using a bioassay conducted on laboratory animals exposed to increasing concentrations of ZnO (500 µg/g, 1000 µg/g, 5000 µg/g, 10000 µg/g, 15000 µg/g).Initial results show that the presence of metallic nanoparticles to ZnO growth caused an inhibition dosedependent in the shell diameter. The growth inhibition is also represented by a reduction dose - dependent on the weight of organs especially Soft (digestive gland and kidney), and reduced dose - dependent consumption rate and dry weight of the waste and compartmental disturbances were noted
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    TOXICITY OF TWO PESTICIDES(SEKATOR AND PROSARO EC 250) INDUCED ALTERATIONS IN LIVER HISTOPHYSIOLOGICAL AND BIOCHEMICAL MARKERS IN MALE RABBITS(Oryctolagus Cuniculus)
    (2022-06-27) Debabsa, R; Grara, N; Khaldi, F; Bouzahouane, H; Guezgouz, N; Gheid, A.
    Sekator and Prosaro EC 250 are common and widely used pesticides in agriculture in Algeria, but the health toxic effects limit their use. Therefore, the present study was undertaken to highlight the toxic effects of Sekator and Prosaro EC 250 on the liver biochemical markers, including serum total bilirubin, and enzymatic activity of transaminases (Aspartate aminotransferase (AST) and alanine aminotransferase (ALT)), and liver histology in male rabbits (Oryctolagus cuniculus).A total of forty-two male rabbits were randomely allocated into seven groups of six animals each, including control group (G1), three groups treated orally with three doses of sekator (mg/kg body weight (bw)), namely G2 (0.213mg/kg bw), G3 (0.426mg/kg bw) and G4 (1.066 mg/kg bw), and three groups treated orally with three doses of prosaro EC 250: G5 (0.093mg/kg bw), G6 (0.186 mg/kg bw) and G7 (0.465 mg/kg bw) for three weeks (21 days).Results showed a significant (P <0.001) increase in the liver absolute and relative weights in 1.066mg/kg bw sekator,and in 0.186 mg/kg and 0.465 mg/kg bw Prosaro treated animals compared with controls. Additionally, the enzymatic activity of transaminases (AST / ALT) and the level of serum total bilirubin were significantly increased (P < 0.001) in the high doses of both pesticides.The biochemical alterations of the liver induced by sekator and prosaro were supported by the histopathological observations showing venous dilation, inflammation, inflammatory infiltrates,congestion in the portal space, ballooned, vacuolated and necrotic hepatocytes, and sinusoidal dilatation