TOXICITY OF TWO PESTICIDES(SEKATOR AND PROSARO EC 250) INDUCED ALTERATIONS IN LIVER HISTOPHYSIOLOGICAL AND BIOCHEMICAL MARKERS IN MALE RABBITS(Oryctolagus Cuniculus)

Abstract

Sekator and Prosaro EC 250 are common and widely used pesticides in agriculture in Algeria, but the health toxic effects limit their use. Therefore, the present study was undertaken to highlight the toxic effects of Sekator and Prosaro EC 250 on the liver biochemical markers, including serum total bilirubin, and enzymatic activity of transaminases (Aspartate aminotransferase (AST) and alanine aminotransferase (ALT)), and liver histology in male rabbits (Oryctolagus cuniculus).A total of forty-two male rabbits were randomely allocated into seven groups of six animals each, including control group (G1), three groups treated orally with three doses of sekator (mg/kg body weight (bw)), namely G2 (0.213mg/kg bw), G3 (0.426mg/kg bw) and G4 (1.066 mg/kg bw), and three groups treated orally with three doses of prosaro EC 250: G5 (0.093mg/kg bw), G6 (0.186 mg/kg bw) and G7 (0.465 mg/kg bw) for three weeks (21 days).Results showed a significant (P <0.001) increase in the liver absolute and relative weights in 1.066mg/kg bw sekator,and in 0.186 mg/kg and 0.465 mg/kg bw Prosaro treated animals compared with controls. Additionally, the enzymatic activity of transaminases (AST / ALT) and the level of serum total bilirubin were significantly increased (P < 0.001) in the high doses of both pesticides.The biochemical alterations of the liver induced by sekator and prosaro were supported by the histopathological observations showing venous dilation, inflammation, inflammatory infiltrates,congestion in the portal space, ballooned, vacuolated and necrotic hepatocytes, and sinusoidal dilatation