Browsing by Author "KHALDI, F"

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    Anti-oxidant activity research of enzymatic biomarkers in protozoa (Paramecium sp.) under stress by manufactured nanoparticles
    (2017-06-28) KHALDI, F; GRARA, N
    The production of nanoparticles (NPs) is increasing rapidly for electronics, chemistry and biology applications. This interest is due to the very small size of NPs which provides them with many interesting properties such as rapid diffusion, high specific surface areas, reactivity in liquid or gas phase and a size close to bio-macromolecules. However, these extreme abilities might be a problem when considering a potentially uncontrolled exposure to the environment. For instance, nanoparticles might be highly mobile and rapidly transported in the environment or inside the body through a water or air pathway. Accordingly, the very fast development of these new synthetic nanomaterials raises questions about their impact on the environment and human health. We have studied the impact of manufactured nanoparticles (ZnO) on ciliated microorganisms (Paramecium sp.).Principles results show that the ZnO presence affects the paramecia growth. On the metabolic level, ZnO causes a disturbance in the rate of total protein and total carbohydrates. Regarding the biomarkers we identified disruption of glutathione (GSH)and catalase (CAT)activity. This study shows the importance of a model species for assessing the ZnO toxicity in laboratory tests. It is clear that ZnO is the oxidative stress source, which results in a stimulation of growth in Paramecium sp.,a disturbance in the total protein rate, carbohydrates, glutathione and catalase activity
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    DETERMINATION OF THE BIOACTIVE COMPOUNDS, ANTIOXIDANT AND ANTIFUNGAL ACTIVITIES OF DIFFERENT EXTRACTS OF Marrubium vulgare L.
    (2021-02-16) MENAIAIA, K; KHALDI, F; OUAHAB, A; BENSOUICI, C.
    The current work concerns a medicinal plant Marrubium vulgare L. grown in Souk Ahras region (Northeastern of Algeria). Widely used by the natives of this region for their extraordinary therapeutic properties such as: antioxidant, antiinflammatory, antibacterial and analgesic activities. The aims of this study were to evaluate the phenolics, flavonoids and tannins composition, and antioxidant activities of hexane, dichloromethane, acetone and methanol extracts from leaves of Marrubium vulgare L. by using different methods. The extracts were a good source of phenolics and flavonoids. Acetonic extract from the aerial part of M. vulgare had the highest total phenolic and flavonoid contents with 171.45±3.38 µg GAE mg -1 E and 78.33±0.29 µg QE mg-1 E, respectively. While the hexane extract had the most condensed tannins content with 90±0.01 µg CE mg -1 E. The antioxidant activity showed the good antioxidant capacity of extracts (dichloromethane, acetone and methanol) in DPPH, ABTS, β-Carotene, CUPRAC, Phenanthroline and GOR assays. While the hexane extract showed the lowest antioxidant activity in DPPH, β-Carotene and GOR assay. Weak antioxidant activity in Reducing power was observed for all extracts with >200 µg/mL. On the other hand, these extracts showed no activity in Ferrous ions chelating assay, exceptionally the methanol extract which showed a very weak activity >800 µg/mL. The results showed that the concentrations of TPC, TFC and CTC were not significantly different (p > 0.05). However, a highly significant difference in all antioxidant activities was observed (p < 0.001) except Phenanthroline activity which presented a non-significant difference (p > 0.05). We have also tested the effect of the different extracts against two phytopathogenic fungi strains by the disc-diffusion method. The inhibitory effect were important for the two tested strains. The extracts of M. vulgare have important antifungal activity. Statistical analysis indicates no significant differences at P > 0.05. Based on These results, it is right to conclude that M. vulgare is an important source of bioactive compounds with antioxidant and antifungal properties.
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    Mesure et contrôle des propriétés de quelques eaux souterraines de l’agglomération d’Ouled Driss, Algérie
    (2018-04-03) KHALDI, F; SMATI, H; GRARA, N; SMATI, N; MAIZI, N; BOUKEHILI, K; GHEID, A.
    Measuring and controlling groundwater properties in Ouled Driss, Algeria Ain Nechema, Ain Souda, Ain Ras Oued, and Ain Maa Bared are important sources of drinking water for the inhabitants of Ouled Driss (Souk Ahras Province, in northeastern Algeria). This article is a qualitative and quantitative study of the physicochemical and bacteriological properties of the water, based on Algerian potability standards and the regulations in force, intended to ensure consumers’ health and well-being. The results of the physicochemical analysis showed that these waters are mineralized with a high content of bicarbonate and calcium, compared with other ions. The results meet Algerian drinking water standards, except for their elevated levels of lead and sodium. The results of the bacteriological analysis showed the presence of several fecal contamination indicators, including total coliforms, fecal coliforms, and fecal streptococci. Several sources of pollution are the cause of this degradation in water quality: waste discharge from the urban area close to the springs and agricultural activities such as cattle breeding