DETERMINATION OF THE BIOACTIVE COMPOUNDS, ANTIOXIDANT AND ANTIFUNGAL ACTIVITIES OF DIFFERENT EXTRACTS OF Marrubium vulgare L.

Abstract

The current work concerns a medicinal plant Marrubium vulgare L. grown in Souk Ahras region (Northeastern of Algeria). Widely used by the natives of this region for their extraordinary therapeutic properties such as: antioxidant, antiinflammatory, antibacterial and analgesic activities. The aims of this study were to evaluate the phenolics, flavonoids and tannins composition, and antioxidant activities of hexane, dichloromethane, acetone and methanol extracts from leaves of Marrubium vulgare L. by using different methods. The extracts were a good source of phenolics and flavonoids. Acetonic extract from the aerial part of M. vulgare had the highest total phenolic and flavonoid contents with 171.45±3.38 µg GAE mg -1 E and 78.33±0.29 µg QE mg-1 E, respectively. While the hexane extract had the most condensed tannins content with 90±0.01 µg CE mg -1 E. The antioxidant activity showed the good antioxidant capacity of extracts (dichloromethane, acetone and methanol) in DPPH, ABTS, β-Carotene, CUPRAC, Phenanthroline and GOR assays. While the hexane extract showed the lowest antioxidant activity in DPPH, β-Carotene and GOR assay. Weak antioxidant activity in Reducing power was observed for all extracts with >200 µg/mL. On the other hand, these extracts showed no activity in Ferrous ions chelating assay, exceptionally the methanol extract which showed a very weak activity >800 µg/mL. The results showed that the concentrations of TPC, TFC and CTC were not significantly different (p > 0.05). However, a highly significant difference in all antioxidant activities was observed (p < 0.001) except Phenanthroline activity which presented a non-significant difference (p > 0.05). We have also tested the effect of the different extracts against two phytopathogenic fungi strains by the disc-diffusion method. The inhibitory effect were important for the two tested strains. The extracts of M. vulgare have important antifungal activity. Statistical analysis indicates no significant differences at P > 0.05. Based on These results, it is right to conclude that M. vulgare is an important source of bioactive compounds with antioxidant and antifungal properties.