Conference papers

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    BODY GROWTH MONITORING OF SMALL RUMINANTS IN THE NORTH-EASTERN ALGERIA
    (2022) REBIAI S , BOUDECHICHE L , OUENNES H , DOUH M
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    Development of MALDI-TOF mass spectrometry for the identification of lice isolated from farm animals
    (EDP Sciences, 2020-04-30) Basma Ouarti; Maureen Laroche; Souad Righi; Mohamed Nadir Meguini; Ahmed Benakhla; Didier Raoul; Philippe Parola
    Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is now routinely used for the rapid identification of microorganisms isolated from clinical samples and has been recently successfully applied to the identification of arthropods. In the present study, this proteomics tool was used to identify lice collected from livestock and poultry in Algeria. The MALDI-TOF MS spectra of 408 adult specimens were measured for 14 species, including Bovicola bovis, B. ovis, B. caprae, Haematopinus eurysternus, Linognathus africanus, L. vituli, Solenopotes capillatus, Menacanthus stramineus, Menopon gallinae, Chelopistes meleagridis, Goniocotes gallinae, Goniodes gigas, Lipeurus caponis and laboratory reared Pediculus humanus corporis. Good quality spectra were obtained for 305 samples. Spectral analysis revealed intra-species reproducibility and inter-species specificity that were consistent with the morphological classification. A blind test of 248 specimens was performed against the in-lab database upgraded with new spectra and validated using molecular tools. With identification percentages ranging from 76% to 100% alongside high identification scores (mean = 2.115), this study proposes MALDI-TOF MS as an effective tool for discriminating lice species.
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    Investigation of flukes (Fasciola hepatica and Paramphistomum sp.) parasites of cattle in north-eastern Algeria
    (Polish Parasitological Society, 2021-07-01) Mohamed Nadir MEGUINI; Souad RIGHI; Mehdi BOUCHEKHCHOUKH; Shehrazed SEDRAOUI; Ahmed BENAKHLA
    Fasciolosis due to Fasciola hepatica is one of the dominant pathologies in Algeria. On the other hand, gastroduodenal paramphistomosis are little studied and little known. Our work consisted of an epidemiological survey in the Souk-Ahras region to investigate these two parasites in cattle. Regarding the epidemiological investigation, it took place in the Souk-Ahras abattoirs, where 530 cattle were inspected for the presence of both parasites. The prevalence of fasciolosis was 12.3%, while the prevalence of paramphistomosis was 7.9% in cattle. Elderly animals were more infected than those under two years of age and females were more infected than males and for both parasites. Therefore, it is important to take into consideration both parasitic diseases and appropriate control measures are strongly recommended to improve cattle productivity.