Journal Articles

Permanent URI for this collectionhttps://dspace.univ-soukahras.dz/handle/123456789/240

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Start date: 2018End date: 2019

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    Green-extraction of essential oil of the species Ruta chalepensis L.: gas chromatography-mass spectroscopy-infra red analysis and response surface methodology optimization
    (De Gruyter Poland Bogumiła Zuga 32A Str. 01-811 Warsaw, Poland, 2019) DJERMANE Mohamed 1, 1, 2, Noureddine 3; ABIDI Abdenabi; CHROUDA Amani; GHERRAF Noureddine
    The objective of the present study was the optimization of the parameters affecting the hydrodistillation of Ruta chalepensis L. essential oil using response surface design type BoxBehnken. After an appropriate choice of three parameters, 15 experiments were performed leading to a mathematical second-degree model relating the response function (yield of essential oil) to parameters and allowing a good control of the extraction process. The realization of the experiments and data analysis was carried out by response surface methodology (RSM). A deduced second-order polynomial expression was used to determine the optimal conditions necessary to obtain a better essential oil yield. These optimized operating conditions were: a granulometry of 2 mm, a condensation-water flow rate of 3.4 mL/min and an extraction time of 204 min. Analysis of variance (ANOVA) indicates that the generated second-order polynomial model was highly significant with R2=0.9589 and P<0.006. The gas chromatography-mass spectrometry analysis of essential oil extracted from the Ruta chalepensis L. aerial parts revealed the presence of 2-undecanone, 2-nonanone and 2-decanone as major components.
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    Editor's choice Water Extract of Tamarix gallica L. as effective Agents against C6 and HeLa tumor cell lines
    (De Gruyter Poland Bogumiła Zuga 32A Str. 01-811 Warsaw, Poland, 2019) Fellah Ouroud 1, 2, 3,; Hameurlaine Samir; Djermane Mohamed; Tahar Wafa; et al
    The aerial parts of Tamarix gallica L. taken from three different climatic stages in Algeria (Oum El Bouaghi: Semi-arid, ElTaref: Humid, and Ouargla: Arid) were extracted using boiling distilled water. The crude extracts were subjected to total phenolics and flavonoids quantifications in addition to anti-proliferative assessment against two tumor cell lines namely rat brain tumor (C6) and human cervix carcinoma (HeLa) using BrdU (bromo-deoxyuridine) ELISA(Enzyme-linked immune-sorbent assay) and xCELLigence assay. The total phenolics yield was found to range between 16.14 and 39.32 mg GAE (Gallic acid equivalent)/g of extract and a flavonoids yield ranging between 16.51 and 20.35 mg QE(quercetin equivalent)/g of extract. The various phenolics were identified using HPLC-TOF/MS to highlight hesperidin and rosmarinic Acid as major components. Moreover, the extracts exhibited different levels of antitumor potency against C6 and HeLa cell lines depending upon the climatic stage and the concentration. A good cytotoxic effect was recorded with the species collected from the humid region at 250 µg/mL. On the contrary, the other extracts revealed a weak activity for both tests.
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    Assessment of Alkaloid Content and Antibacterial Activity of Hyoscyamus Albus and Hyoscyamus Muticus Collected in Two Different Climatic Regions in Algeria
    (SEVAS EDUCATIONAL SOC, 2019-03-15) Kebaili Zoubir; Hameurlaine Samir; Fellah Ouroud; Djermane Mohamed; Gherraf Noureddine; Zellagui Amar; Abidi Abdenabi; Derouiche Kamel
    Medicinal plants are considered as a very important source of secondary metabolites and therefore bioactive molecules. Our comparative study deals with two species: Hyoscyamus albus and Hyoscyamus muticus harvested in two different climate regions namely: Arris (semi-arid, wilaya of Batna) and Djanet (arid, wilaya of Illizi), respectively. The phytochemical screening revealed that aerial parts of the two species contain almost all classes of secondary metabolites such as flavonoids, quinones, anthraquinones, saponins, tannins, coumarins and alkaloids. The quantitative assessment of alkaloids in hydroalcoholic extracts of the two plants showed that the total alkaloid content varies depending upon the species and climate conditions. The alkaloid level was found to be 0.51% and 0.32% in Hyoscyamus albus and Hyoscyamus muticus respectively. Moreover, the antibacterial test vis-à-vis some pathogen bacteria such as: Escherichia coli, Staphylococcus aureus, Staphylococcus aureus and Pseudomonas aeruginosa showed that both alkaloid crude extracts and hydroalcoholic extracts are moderately active against the tested bacterial strains.
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    Eco-Extraction of Essential Oil of the Species Salvia Officinalis L. Systematic Characterization and Parametric Modeling
    (EURESIAN PUBLICATIONS, 2018-07-23) DJERMANE Mohamed; Abidi Abdenabi; Cheraif Imed; Toubal Abdelaziz; Noureddine Gherraf
    In this study, the response surface methodology (RSM) was used to optimize the hydrodistillation process of Algerian Salvia officinalis essential oil. The effects of grain size, condensing flow rate and extraction time on oil yield were studied. A 3-factor design by the BoxBehnken method was used to produce factor combinations, from the response surface analysis. A second-order polynomial expression was deduced and used to determine the best yield efficiency of extracted oil according to the optimal conditions. Analysis of variance (ANOVA) indicates that the generated second-order polynomial model was highly significant with R2 = 0.9589 and P <0.006. Optimum oil yield conditions (0.98%) were estimated at a particle diameter of 2mm, a condensation flow rate of 1.4mL/min of and an extraction time of 210min. The essential oil extracted from the sage leaves by hydrodistillation was analyzed by GC/MS, to afford α.-Thujone, camphor, 1,8- cineole and β.-Thujone as main components.