HPLC Chromatographic Analysis Antioxidant activity of seeds some varieties of prickly pear (Opuntia ficus-indica L.) from the Sidi-Fredj souk Ahras Algeria

Abstract

The study was conducted on seeds of three varieties of Opuntia ficus-indica(O. ficus-indica), harvested from different regions of Souk Ahrasinnorth-East of Algeria(Sidi-Fredj,Taoura and Drea).The analysis consisted of antioxidants extraction, following the solid-liquid extraction with ethanol 40% (v/v), their quantification and determination of their antioxidant activity by two methods (reducing power and DPPH (2-2 diphenyl 1-picryl hydrazyl)Test). Results show that total phenolic content (TPC) ranged from 71,01to 90,79mg GAE(Gallic Acid Equivalent)/100g Extract.The Sidi-Fredj variety has the best rate followedby the Taoura and Drea ones. Concerning, flavonoids and tannins, the Taoura variety contains higher concentrations corresponding to 2.67 mg QE(Quercetin equivalent)/100g and 6.60mg CE(Cyanidin equivalent)/100g. Activitiesof extracts of three varieties show similar performance; the EC50for the reduction of ferric iron 0.05g/mL for all extracts, whereas the EA is more important in Sidi-Fredj and Drea seeds extracts. Regarding the scavenging of DPPH, Drea and Sidi-Fredj varieties show the highest capacity equals to 0.20g/mL. Linear correlations between the different studied activities and some antioxidants (flavonoids, tannins) rates were noticed; indicating their participation in the obtained effect. Analysis of the oil by HPLC revealed the presence of phenolic compounds including gallic acid and a form of vitamin E (α-tocophérol), capable of expressing biological activities; Anti-oxidants and vitamins. GC (Gas Chromatography)analysis showed that prickly pear oil was a major source of essential fatty acids (C18:2 and C18:3).The anti-free radical activity of the seed was evaluatedthrough a DPPH chemical test. The results obtained show a hight reducing activity of our extract.