Department of Biology

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Author: search.filters.author.Bouzahouane, H

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    Multi-biomarkers approach to the assessment of the southeastern Mediterranean Sea health status: Preliminary study on Stramonita haemastoma used as a bioindicator for metal contamination
    (2018-06-25) Bouzahouane, H; Barour,C; Sleimi, N; Ouali, K.
    The present study aimed to evaluate the responses of different biochemicals parameters associated with environmental pollution in the digestive gland of the gastropod mollusc Stramonita haemastoma. Physiochemical parameters and trace metal elements (Copper (Cu), Zinc (Zn), Chromium (Cr), Cadmium (Cd) and Lead (Pb)) were measured in seawater. Spatiotemporal variations in reduced glutathione (GSH), malondialdehyde (MDA) and metallothionein (Mt) as well as the specific activities of glutathione Stransferase (GST) and catalase (CAT) were evaluated in digestive gland of this species during a one-year period in 2013e2014. Samples collection was conducted at three sites. The results obtained showed seasonal fluctuations in GST and CAT activities and in the rate of Mt content. In addition, intersite variations in GSH, MDA, Mt and CAT were recorded in individuals. Also, trace metal elements concentrations determined by season in the digestive gland revealed spatial and temporal variations for Cu and Zn but they are below the limit of detection for Cd and Pb. The highest values were generally recorded in spring for Cu and in winter for Zn. In this first regional study using in S. haemastoma as a model, the biomarkers measured were seen to be inducible parameters to evaluate the health state of the organism and the overall quality of the study sites.
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    TOXICITY OF TWO PESTICIDES(SEKATOR AND PROSARO EC 250) INDUCED ALTERATIONS IN LIVER HISTOPHYSIOLOGICAL AND BIOCHEMICAL MARKERS IN MALE RABBITS(Oryctolagus Cuniculus)
    (2022-06-27) Debabsa, R; Grara, N; Khaldi, F; Bouzahouane, H; Guezgouz, N; Gheid, A.
    Sekator and Prosaro EC 250 are common and widely used pesticides in agriculture in Algeria, but the health toxic effects limit their use. Therefore, the present study was undertaken to highlight the toxic effects of Sekator and Prosaro EC 250 on the liver biochemical markers, including serum total bilirubin, and enzymatic activity of transaminases (Aspartate aminotransferase (AST) and alanine aminotransferase (ALT)), and liver histology in male rabbits (Oryctolagus cuniculus).A total of forty-two male rabbits were randomely allocated into seven groups of six animals each, including control group (G1), three groups treated orally with three doses of sekator (mg/kg body weight (bw)), namely G2 (0.213mg/kg bw), G3 (0.426mg/kg bw) and G4 (1.066 mg/kg bw), and three groups treated orally with three doses of prosaro EC 250: G5 (0.093mg/kg bw), G6 (0.186 mg/kg bw) and G7 (0.465 mg/kg bw) for three weeks (21 days).Results showed a significant (P <0.001) increase in the liver absolute and relative weights in 1.066mg/kg bw sekator,and in 0.186 mg/kg and 0.465 mg/kg bw Prosaro treated animals compared with controls. Additionally, the enzymatic activity of transaminases (AST / ALT) and the level of serum total bilirubin were significantly increased (P < 0.001) in the high doses of both pesticides.The biochemical alterations of the liver induced by sekator and prosaro were supported by the histopathological observations showing venous dilation, inflammation, inflammatory infiltrates,congestion in the portal space, ballooned, vacuolated and necrotic hepatocytes, and sinusoidal dilatation
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    Multi-biomarkers approach to the assessment of the southeastern Mediterranean Sea health status: Preliminary study on Stramonita haemastoma used as a bioindicator for metal contamination
    (2018-05-25) Bouzahouane, H; Barour, C; Sleimi, N; Ouali, K.
    The present study aimed to evaluate the responses of different biochemicals parameters associated with environmental pollution in the digestive gland of the gastropod mollusc Stramonita haemastoma. Physiochemical parameters and trace metal elements (Copper (Cu), Zinc (Zn), Chromium (Cr), Cadmium (Cd) and Lead (Pb)) were measured in seawater. Spatiotemporal variations in reduced glutathione (GSH), malondialdehyde (MDA) and metallothionein (Mt) as well as the specific activities of glutathione Stransferase (GST) and catalase (CAT) were evaluated in digestive gland of this species during a one-year period in 2013e2014. Samples collection was conducted at three sites. The results obtained showed seasonal fluctuations in GST and CAT activities and in the rate of Mt content. In addition, intersite variations in GSH, MDA, Mt and CAT were recorded in individuals. Also, trace metal elements concentrations determined by season in the digestive gland revealed spatial and temporal variations for Cu and Zn but they are below the limit of detection for Cd and Pb. The highest values were generally recorded in spring for Cu and in winter for Zn. In this first regional study using in S. haemastoma as a model, the biomarkers measured were seen to be inducible parameters to evaluate the health state of the organism and the overall quality of the study sites