Department of Biology
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Item COMPARATIVE STUDY BETWEEN THE TOXICITY OF ALUMINIUM CHLORIDE AND MERCURIC CHLORIDE ON BIOCHEMICAL MARKERS AND LIPID PROFILES IN WISTAR RATS(2022-06-07) Sayah, S; Khaldi, F; Araar, S; Chaib, S; Gheid, A.This study was designed to compare the between the toxic of aluminum chloride (AlCl3) and mercuric chloride (HgCl2) on biochemical markers of liver function, and lipid profiles in Wistar rats. Forty-two male albino Wistar rats were equally divided into three main groups as untreated control (n= 6) group and two treated (n = 6) groups which then were subdivided each into three different subgroups depending on the metal type and doses, namely AlCl3 at doses 7.6, 12.66, and 38 mg/kg body weight (bw), and HgCl2 at doses 0.1, 0.2, and 0.4 mg/kg (bw). Treatments were given to rats orally for 28 days. Results showed a decrease in body weight, and an increase in relative and absolute liver weights, in addition to a significant increase in the levels of serum glucose, bilirubin (total and direct), cholesterol and triglycerides, and the enzymatic activities of transaminases (TGO, TGP) and alkaline phosphatase (PAL), and conversely a decreased level of total proteins in Al and Hg treated animals compared to the controls. The biochemical alterations were supported by the histopathological evaluation of the liver, showing vascular congestion, sinusoid disintegration, centrilobular vein disintegration, and inflammatory cell infiltration. In conclusion, AlCl3 and HgCl2 have induced liver dysfunction, and HgCl2 was proved to be a very toxic metaItem TOXICITY OF TWO PESTICIDES(SEKATOR AND PROSARO EC 250) INDUCED ALTERATIONS IN LIVER HISTOPHYSIOLOGICAL AND BIOCHEMICAL MARKERS IN MALE RABBITS(Oryctolagus Cuniculus)(2022-06-27) Debabsa, R; Grara, N; Khaldi, F; Bouzahouane, H; Guezgouz, N; Gheid, A.Sekator and Prosaro EC 250 are common and widely used pesticides in agriculture in Algeria, but the health toxic effects limit their use. Therefore, the present study was undertaken to highlight the toxic effects of Sekator and Prosaro EC 250 on the liver biochemical markers, including serum total bilirubin, and enzymatic activity of transaminases (Aspartate aminotransferase (AST) and alanine aminotransferase (ALT)), and liver histology in male rabbits (Oryctolagus cuniculus).A total of forty-two male rabbits were randomely allocated into seven groups of six animals each, including control group (G1), three groups treated orally with three doses of sekator (mg/kg body weight (bw)), namely G2 (0.213mg/kg bw), G3 (0.426mg/kg bw) and G4 (1.066 mg/kg bw), and three groups treated orally with three doses of prosaro EC 250: G5 (0.093mg/kg bw), G6 (0.186 mg/kg bw) and G7 (0.465 mg/kg bw) for three weeks (21 days).Results showed a significant (P <0.001) increase in the liver absolute and relative weights in 1.066mg/kg bw sekator,and in 0.186 mg/kg and 0.465 mg/kg bw Prosaro treated animals compared with controls. Additionally, the enzymatic activity of transaminases (AST / ALT) and the level of serum total bilirubin were significantly increased (P < 0.001) in the high doses of both pesticides.The biochemical alterations of the liver induced by sekator and prosaro were supported by the histopathological observations showing venous dilation, inflammation, inflammatory infiltrates,congestion in the portal space, ballooned, vacuolated and necrotic hepatocytes, and sinusoidal dilatationItem TOXICOLOGICAL EVALUATION OF A MONOAMMONIUM PHOSPHATE FERTILIZER IN RATS FOLLOWING 30 DAYS OF REPEATED ORAL EXPOSURE(2022-07-06) Araar, S; Khaldi, F; Sayah, S; Chaib, S; Gheid, A.The present study was undertaken to evaluate the subacute toxicity of a synthetic fertilizer widely used in agriculture (Phosfert®, monoammonium phosphate NH4H2PO4 (MAP)) on some haematological and biochemical profiles as well as liver and kidney histology in Wistar rats. MAP was administered to rats orally at 200, 400, and 800mg/kg of body weight doses for 30 days. Results showed decreased body weight and a significant increase in liver and kidney relative weights in MAP-treated rats. In addition, hematotoxicity effect of high doses of MPA was evidenced by decreased levels of red blood cells (RBC), haemoglobin (Hb) and hematocrit (Ht) along with increased levels of white blood cell (WBC) counts. Further, hepatic and renal markers and lipid profiles were markedly increased however; total proteins and albumin levels were considerably decreased in MAP treated rats as compared with controls. These effects were supported by the liver and kidney histopathological evaluations. Conclusively, the study proved that the long-term use and at higher doses of MAP may cause adverse hepatic and renal effectsItem Calcium Nitrate Toxicity on Rat Liver and Kidney Functions: A Biochemical and Histopathological Evaluation(2022-06-30) Araar, S; Khaldi, F; Sayah, S; Chaib, S; Gheid, A.Calcium NitrateTetrahydrate is a wide-used nitrogen fertilizer in Algerian agriculture. The present study was aimed to examine the toxic effects of calcium nitrate on kidney and liver functional biochemical markers. Twenty-eight male albino wistar male rats were divided into three treated groups receiving orally 200, 400 and 800mg/kg of calcium nitrate, and one untreated control group. Results showed a dose- dependent increase in kidney and liver relative weights, serum levels of glucose, cholesterol, triglyceride, blood urea, creatinine, and uric acid, and enzymatic activity of transaminases and alkaline phosphatase. However, serum protein and albumin levels were significantly decreased in a dose dependent manner as compared with those of control group. In addition, hepatic and renal histological changes were evidenced by hepatocyte degeneration, necrosis, dilation and sinusoid congestion, atrophy of glomeruli, vascular congestion, and infiltration of inflammatory cells. It is noteworthy that these adverse stress effects were higher in 400 and 800 mg/kg calcium nitrate treated rats than those treated with 200mg/kg and control group. In conclusion, the study proved the effective ability of subacute exposure of calcium nitrate to induce liver and kidney stress dysfunctions